BiophysicsbioRxivPreprint
Kinetic Interval Measurement: A Tool to Characterize Thermal Reversion Dynamics of Light-switchable Fluorescent Proteins
T. von Trotha, R. Johr, J. Fischer…·C. Kluger
The paper introduces KIM, a method to measure thermal reversion dynamics of light-switchable proteins using a microplate reader, showing its ability to determine reversion half-life and sensitivity to temperature and imidazole concentration.
DOI 10.1101/2021.02.20.432101