Live-cell imaging reveals the trade-off between target search flexibility and efficiency for Cas9 and Cas12a
L. Olivi, C. Bagchus, V. Pool, E. Bekkering, K. Speckner, W. Wu, K. Martens, J. van der Oost, R. Staals, J. Hohlbein
The paper uses live-cell imaging to compare the target search efficiency of Cas9 and Cas12a variants, finding that LbdCas12a is faster and more efficient than SpydCas9.
bioRxiv · Nov 16, 2023Biophysics