J. van der Oost

The paper uses live-cell imaging to compare the target search efficiency of Cas9 and Cas12a variants, finding that LbdCas12a is faster and more efficient than SpydCas9.

This study characterizes a type III-B CRISPR-Cas system in Haliangium ochraceum, revealing a proteolytic cascade involving SAVED-CHAT and PCaspase that activates immune responses against RNA targets.

This paper develops a CRISPR-Cas12a system for efficient genome engineering in Clostridium beijerinckii NCIMB 8052, achieving single-gene knockouts and multiplex editing.

This study compares metagenomic data from sponge microbiomes to identify novel biosynthetic gene clusters and their conservation across species.

This study identifies key regions in type III CRISPR-Cas systems for RNA targeting and cOA production, leading to the development of the SCOPE diagnostic tool for SARS-CoV-2 detection.

This paper identifies a novel arsenite methyltransferase in Thermus thermophilus and develops a hyperthermophilic Cas9-based genome-editing tool to study its role in arsenic resistance.

This paper introduces SIBR-Cas, a CRISPR-based genome engineering tool that works universally in bacteria without host factors or exogenous recombinases, enabling gene knockouts in species with poor homologous recombination.

This paper introduces Ago-PAINT, a method that accelerates DNA-PAINT imaging by using Argonaute protein to enhance imager strand binding, achieving 10x faster acquisition without compromising resolution.

This study shows Argonaute proteins efficiently search for targets by gliding over secondary structures and bypassing protein barriers through lateral diffusion and intersegmental jumps.

This study identifies and characterizes ThermoCas9, a thermostable Cas9 variant active across a broad temperature range, and develops tools for bacterial genome editing and silencing at elevated temperatures.