Author

R. Staals

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 1 Bioengineering· 1 Biophysics· 1

Signature Assays, Organisms & Methods

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Organism:bacteria (other)· 2 Research Focus:cell signaling & trafficking· 2 Approach:experimental assay· 2 Assay:CRISPR screen· 1 Organism:E. coli· 1 Assay:fluorescence / live-cell microscopy· 1 Research Focus:gene regulatory network inference· 1 Approach:genetic perturbation / CRISPR· 1

Frequent Co-Authors

J. van der Oost· 3 shared C. Patinios· 2 shared A. Arifah· 1 shared A. Bardis· 1 shared A. Barendrecht· 1 shared A. M. Amieiro· 1 shared B. Perez· 1 shared C. Bagchus· 1 shared

Activity by Year

2021-2024 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 25 mutual co-author links

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3 shared papersBiophysics

J. van der Oost

Mutual co-authors in cluster

Author profile →

3 papers

Recent·Most cited

Live-cell imaging reveals the trade-off between target search flexibility and efficiency for Cas9 and Cas12a

L. Olivi, C. Bagchus, V. Pool, E. Bekkering, K. Speckner, W. Wu, K. Martens, J. van der Oost, R. Staals, J. Hohlbein

The paper uses live-cell imaging to compare the target search efficiency of Cas9 and Cas12a variants, finding that LbdCas12a is faster and more efficient than SpydCas9.

Nucleic Acids Research · Apr 22, 2024Peer-reviewedBiophysics

Type III-B CRISPR-Cas signaling-based cascade of proteolytic cleavages

J. Steens, J. P. Bravo, C. R. P. Salazar, C. Yildiz, A. M. Amieiro, S. Kostlbacher, S. H. P. Prinsen, C. Patinios, A. Bardis, A. Barendrecht, R. A. Scheltema, T. J. G. Ettema, J. van der Oost, D. W. Taylor, R. Staals

This study characterizes a type III-B CRISPR-Cas system in Haliangium ochraceum, revealing a proteolytic cascade involving SAVED-CHAT and PCaspase that activates immune responses against RNA targets.

bioRxiv · Jun 23, 2023 · PreprintBiochemistry

SIBR-Cas enables host-independent and universal CRISPR genome engineering in bacteria

C. Patinios, S. Creutzburg, A. Arifah, B. Perez, C. Ingham, S. Kengen, J. van der Oost, R. Staals

This paper introduces SIBR-Cas, a CRISPR-based genome engineering tool that works universally in bacteria without host factors or exogenous recombinases, enabling gene knockouts in species with poor homologous recombination.

bioRxiv · Apr 26, 2021Bioengineering