J. Hohlbein

Live-cell imaging reveals the trade-off between target search flexibility and efficiency for Cas9 and Cas12a

The paper uses live-cell imaging to compare the target search efficiency of Cas9 and Cas12a variants, finding that LbdCas12a is faster and more efficient than SpydCas9.

Temporal analysis of relative distances (TARDIS) is a robust, parameter-free alternative to single-particletracking

TARDIS provides a parameter-free alternative to single-particle tracking by analyzing temporal distance distributions to infer particle movement and interactions more robustly than existing methods.

Cooperative action of separate interaction domains promotes high-affinity DNA binding of Arabidopsis thaliana ARF transcription factors

This study investigates how DNA binding and dimerization domains of Arabidopsis ARF transcription factors influence DNA binding affinity and kinetics using single-molecule FRET assays.

Enabling spectrally resolved single-molecule localization microscopy at high emitter densities

This paper presents a low-cost sSMLM implementation that improves spatio-spectral resolution and emitter density using a transmission grating and provides analysis tools.

Probing DNA - transcription factor interactions using single-molecule fluorescence detection in nanofluidic devices

The paper uses single-molecule FRET in nanofluidic devices to study ARF-DBD interactions with DNA, showing monomeric behavior and FRET changes upon binding.

Enabling single-molecule localization microscopy in turbid food emulsions

This paper combines adaptive optics with single-molecule localization microscopy to achieve sub-100 nm resolution imaging of phosvitin in turbid food emulsions.

Extracting transition rates in single-particle tracking using analytical diffusion distribution analysis

This paper introduces anaDDA, a method to extract transition rates from diffusion coefficient distributions in single-particle tracking, showing improved performance in fast regimes and applications to DNA polymerase I in E. coli.

Analyzing engineered point spread functions using phasor-based single-molecule localization microscopy

This paper adapts and evaluates a phasor-based single-molecule localization microscopy algorithm for engineered PSFs, achieving comparable precision to existing methods with improved speed.

Design principles of a minimal auxin response system

The paper identifies a minimal auxin response system in Marchantia polymorpha, showing that a single A-ARF activates gene expression while a B-ARF represses it, defining developmental zones of auxin response.

Direct visualization of native CRISPR target search in live bacteria reveals Cascade DNA surveillance mechanism

The study visualizes Cascade complexes in live bacteria to reveal their DNA surveillance mechanism and how they detect invading DNA.

An open microscopy framework suited for tracking dCas9 in live bacteria

The paper introduces a low-cost super-resolution microscope for tracking dCas9 in live bacteria and quantifies its binding kinetics.

Substrate conformational dynamics drive structure-specific recognition of gapped DNA by DNA polymerase

This paper investigates how DNA polymerase I recognizes gapped DNA structures through conformational dynamics, revealing a bent DNA structure and using single-molecule FRET and simulations to support the findings.

Phasor based single-molecule localization microscopy in 3D (pSMLM-3D): an algorithm for MHz localization rates using standard CPUs

This paper introduces a fast, model-free single-molecule localization algorithm for 2D and 3D imaging that achieves high localization rates on standard CPUs.

Simple nanofluidic devices for high-throughput, non-equilibrium studies at the single-molecule level

The paper introduces nanofluidic devices for high-throughput single-molecule studies, enabling precise flow profiling and real-time chemical reaction observation.