Evolutionary BiologybioRxiv
Enigmatic Diphyllatea eukaryotes: Culturing and targeted PacBio RS amplicon sequencing reveals a higher order taxonomic diversity and global distribution
R. J. S. Orr, S. Zhao, D. Klaveness…·K. Shalchian-Tabrizi
The paper cultures Diphyllatea strains, amplifies full 18S rRNA genes using PacBio RS II, and identifies three clades, revealing two morphotypes and broader global distribution.
DOI 10.1186/s12862-018-1224-z