Daniel J. Bernard

This paper develops a method to produce highly purified inhibins using mammalian cell expression and tandem affinity purification, achieving high yields and purity with minimal activin contamination.

The paper uses single-nucleus multiomics to study pituitary cell changes during the mouse estrous cycle, finding stage-dependent gene regulatory circuits and validated ETS2-driven gene expression patterns.

The paper identifies four activin-sensitive enhancers upstream of the murine Fshb gene, showing differential regulation by SMAD2/3 and FOXL2 in gonadotropes.

The paper creates a large-scale Consensus Pituitary Atlas to improve reproducibility and enable marker discovery and analysis in pituitary research.

This study shows that Gata4 downregulation in the hypothalamus of Greywick female mice models the metabolic subtype of PCOS by affecting fertility and food intake genes.

This study determines the structure of TGF-beta bound to betaglycan and its signaling receptors, revealing mechanisms of ligand selectivity and signal potentiation.

The paper identifies myostatin as an endocrine hormone that promotes pituitary FSH synthesis and ovarian function, challenging the previous role of activins and highlighting potential fertility risks of myostatin antagonists.

SPEEDI automates single-cell multi-omics analysis by integrating data, inferring batches, and labeling cell types without user input, improving reproducibility and accessibility.

This study identifies Activin E as a TGFβ ligand that signals through ALK7, using canonical activin receptors and SMAD2/3 pathways, similar to Activin C.

The paper introduces CREMA, a framework for mapping cis-regulatory circuits using single-cell multiome data without peak-calling, and validates it using mouse pituitary data.