Pierce Eggan

This study uses time-resolved tmFRET to analyze conformational changes and energetics in the CNBD of SthK, showing that transmembrane domains make the activating conformation more favorable.

This study uses time-resolved tmFRET to analyze conformational changes and energetics in the SthK C-terminal region upon cAMP and cGMP binding, revealing large structural changes with cAMP and partial agonism with cGMP.

This paper develops a fluorescence method to measure protein conformational dynamics and energetics using time-resolved tmFRET and validates it with EPR spectroscopy.