Author

John J. Alvarado

University of PittsburghORCID 0000-0001-6274-5997Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Microbiology· 1 Pharmacology & Toxicology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Organism:human· 2 Research Focus:drug response / drug discovery· 1 Research Focus:protein folding & dynamics· 1 Assay:protein structure· 1

Frequent Co-Authors

Thomas E. SmithgalliD· 2 shared Allen B. ReitziD· 1 shared Catherine E. ThomasiD· 1 shared Colin M. TiceiD· 1 shared Haibin ShiiD· 1 shared Lori A. Emert‐SedlakiD· 1 shared Sherry T. Shu· 1 shared

Activity by Year

2024-2026 · peak 1/yr

Co-Author Collaboration Network

7 top collaborators · 16 mutual co-author links

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2 shared papersMicrobiology

Thomas E. SmithgallORCID

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2 papers

Recent·Most cited

MicrobiologybioRxivPreprintAug 11, 2026

HIV-1 Nef Homodimerization as a Structural Mechanism for Kinase Activation and Small Molecule Inhibitor Action

Catherine E. Thomas, John J. Alvarado, Thomas E. Smithgall

This study shows that HIV-1 Nef homodimerization is critical for kinase activation and that disrupting this interface with small molecules inhibits viral replication and restores immune recognition.

DOI 10.64898/2026.08.10.744017

humanprotein structure

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Pharmacology & ToxicologyCell chemical biologyPeer-reviewedMar 19, 2024

PROTAC-mediated Degradation of HIV-1 Nef Efficiently Restores Cell-surface CD4 and MHC-I Expression and Blocks HIV-1 Replication

Lori A. Emert‐Sedlak, Colin M. Tice, Haibin Shi…·Thomas E. Smithgall

This study develops PROTACs to degrade HIV-1 Nef, restoring CD4 and MHC-I expression and inhibiting HIV-1 replication in T cells and PBMCs.

42 citationstop 3%

humanexperimental assay

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