Içvara Barbier

This paper engineers a multi-step differentiation program in E. coli to generate self-organized spatial patterns through symmetry breaking, paracrine, and autocrine signaling.

The paper analyzes the AC-DC genetic circuit to show it can exhibit diverse multifunctional dynamics, including coexisting oscillations and multistability, challenging traditional views of network constraints.

This study experimentally tests Ohno's hypothesis by evolving fluorescent protein genes in E. coli, finding that double-copy populations show increased mutational robustness and diversity but not accelerated phenotypic evolution.

The paper shows that using the same guide RNA structure for CRISPRi and CRISPRa in E. coli prevents context-dependent effects, ensuring predictable gene circuit behavior.

This study builds a synthetic toggle switch in E. coli to control spatiotemporal gene expression patterns using diffusible molecules and mathematical modeling.