David Colognori

This paper introduces spligation, a method using CRISPR-Csm complexes to generate chimeric RNA in living cells by enabling site-specific RNA cleavage and trans-ligation.

This study identifies a trade-off between PAM recognition breadth and CRISPR-Cas9 editing efficiency, proposing a two-step target capture mechanism to improve genome editing.

The paper develops a CRISPRi-ART system to knock down protein expression in diverse bacteriophages and identifies essential genes, including many with unknown functions.

This paper introduces smLiveFISH, a method for single-molecule live-cell imaging of endogenous RNA using CRISPR-Csm, and identifies two distinct mRNA localization mechanisms.

This study enhances CRISPR-Cas9-mediated HDR efficiency by fusing Cas9 with PRDM9, increasing HDR by three-fold and HDR:indel ratio by five-fold without increasing off-target effects.

This study investigates how cohesin depletion or retention affects X-inactivation and chromosomal interactions, finding that cohesin balance is critical for proper Xi structure and gene silencing.