Alyssa K. Overton

This paper develops and implements wastewater-based surveillance methods to monitor Influenza A H3N2 and respiratory syncytial virus A in Ontario, Canada, enabling real-time tracking of viral lineages and informing public health decisions.

The paper evaluates nine computational tools for identifying SARS-CoV-2 lineages in wastewater sequencing data using simulated samples, finding most tools reliably detect lineages above 5% frequency.

The paper develops a tiled-amplicon sequencing assay to detect and track ToBRFV in municipal wastewater, revealing its widespread distribution in Ontario, Canada.