Sha Huang

The paper maps mesenchymal diversity in the developing human intestine and organoids using single-cell RNA-sequencing and spatial transcriptomics, identifying distinct fibroblast populations and their spatial organization.

This study develops an in vitro method to generate human intestinal organoids containing multiple cell types, including neurons, endothelial cells, and smooth muscle, which functionally mimic gut physiology.

This study develops iPSC-derived lung bud tip progenitor organoids that can be expanded and differentiated into airway or alveolar organoids, showing transcriptional similarity to in vivo progenitors.

The paper uses ChRO-seq to study chromatin dynamics during human small intestinal development, identifying enhancer activity and transcription patterns at HOX gene loci.

This study shows that non-adhesive alginate hydrogels support the growth and differentiation of human intestinal organoids, comparable to Matrigel.

This study induces and engrafts human pluripotent stem cell-derived lung bud tip progenitor-like cells in vitro and in vivo, showing their similarity to native lung tissue and multi-lineage potential.

This study uses human intestinal organoids to show microbial colonization induces complex physiological changes in immature human epithelium, including antimicrobial peptide production and mucus maturation.