Yu-Hwai Tsai

This study examines how human enteroids respond to various injuries, finding limited reactivation of developmental gene programs and highlighting the role of specific signaling pathways.

The paper constructs a developmental cell atlas of the human esophagus and develops in vitro models that recapitulate esophageal architecture, revealing WNT2B's role in regulating basal progenitor self-renewal.

The paper constructs a cellular atlas of the early postnatal human lung and identifies two distinct AT2 cell states linked to inflammatory signaling and clinical interventions.

The paper maps mesenchymal diversity in the developing human intestine and organoids using single-cell RNA-sequencing and spatial transcriptomics, identifying distinct fibroblast populations and their spatial organization.

This study develops an in vitro method to generate human intestinal organoids containing multiple cell types, including neurons, endothelial cells, and smooth muscle, which functionally mimic gut physiology.

The paper creates a spatially-resolved atlas of the developing human lung, identifying a WNT-driven niche that regulates basal cells.

This study develops iPSC-derived lung bud tip progenitor organoids that can be expanded and differentiated into airway or alveolar organoids, showing transcriptional similarity to in vivo progenitors.

The paper identifies a novel secretory-like cell type in the developing human lung that gives rise to pulmonary neuroendocrine cells and multiciliated cells.

The paper identifies EPIREGULIN as a niche factor that enables spatial organization in human intestinal organoids, mimicking the native intestine's structure and gene expression.

The paper identifies R-SPONDIN2-expressing mesenchymal cells as a niche for lung bud tip progenitors using single-cell RNA sequencing and functional experiments.

This study uses scRNA-seq to analyze human intestinal development, identifying cell sources of niche factors and their functional roles in stem cell maintenance.

This study enhances the co-differentiation of intestinal organoids with endothelial cells, showing they share an intestinal-specific transcriptional signature with native cells.

The paper uses human intestinal organoids to create a single-cell transcriptome atlas, revealing molecular mechanisms of cell fate specification in the intestine.

The paper identifies SMAD signaling as a regulator of basal cell fate specification in the developing human lung using single-cell transcriptomics and organoid models.

The paper identifies HNF4 factors as critical for fetal intestinal maturation, showing their role in chromatin accessibility and gene regulation during the embryonic-to-fetal transition.

This study shows CDX2 has different chromatin-binding profiles in embryonic and adult intestines, leading to distinct gene activation patterns and developmental outcomes.

This study shows that non-adhesive alginate hydrogels support the growth and differentiation of human intestinal organoids, comparable to Matrigel.

This study uses human intestinal organoids to show microbial colonization induces complex physiological changes in immature human epithelium, including antimicrobial peptide production and mucus maturation.