Christopher John Schofield

This paper characterizes OXA-57, a class D β-lactamase from Burkholderia pseudomallei, showing its activity against penicillins and cephalosporins, limited carbapenem hydrolysis, and structural insights into its mechanism.

This study identifies MBLAC2 as a nucleolar nuclease essential for co-transcriptional rRNA processing and global transcriptional output.

This study shows 3-BP covalently inhibits NDM-1 and NDM-5 MBLs, restoring carbapenem activity against resistant bacterial strains.

This paper describes a drop on fixed target method for time-resolved serial crystallography, tested with enzymes to improve measurement consistency across facilities.

This study investigates how acyl-enzyme tautomerisation, dynamics, and hydration affect carbapenem antibiotic turnover by OXA-48 β-lactamase using X-ray crystallography and molecular dynamics simulations.

This study uses room-temperature serial crystallography to observe time-dependent ligand dynamics of the β-lactamase CTX-M-15 with avibactam, revealing conformational changes critical for inhibitor reformation.

This study investigates the systemic effects of microbiome-derived carnitine analogues in mice, showing carnitine depletion and mitochondrial dysfunction due to inhibition of carnitine biosynthesis.

This paper identifies JMJD5-ISY1-PRMT6 as a pathway regulating metabolism by hydroxylating ISY1 to inhibit PRMT6, showing its role in liver cancer and potential as a therapeutic target.

This study evaluates the roles of Ldt and PBP inhibition in suppressing Mycobacterium smegmatis using beta-lactams and LdtMt2 inhibitors, finding stronger growth suppression with Ldt inhibition.

This paper introduces a high-throughput activity proteomics pipeline for discovering deubiquitinase inhibitors, identifying potent and selective inhibitors with minimal optimization.

This paper compares the potency of nacubactam and avibactam against OXA-48-like β-lactamases, finding that nacubactam is less potent due to electrostatic repulsion with Arg214.

This study identifies how CSB enhances SNM1A nuclease activity during ICL repair by interacting with its nuclease core and altering substrate conformation.

The paper investigates how cardiac stress regulates Filamin C dimerisation through phosphorylation-modulated interactions with HSPB7, revealing evolutionary conservation of this mechanism.

This study uses dynamical nonequilibrium molecular dynamics simulations to investigate how SARS-CoV-2 main protease dynamically recognizes and responds to substrate binding, revealing key conformational changes and substrate selectivity mechanisms.

This study investigates how the P2' proline residue affects SARS-CoV-2 PLpro catalytic efficiency using molecular dynamics and substrate analysis.

The paper models SARS-CoV-2 Mpro substrate and ligand binding to identify new inhibitor opportunities through computational analysis of binding interactions.

The paper shows that disrupting dNTP homeostasis is synthetically lethal with CDK-induced replication stress in fission yeast, leading to genome instability and cell death.