Lukas Schrangl

This paper introduces FRET-TTB, a method to track transfected biomolecules in living cells, showing it can measure conformational changes and localization with single-molecule resolution.

The paper quantifies mechanical forces on TCR:pMHC interactions in CD4+ T-cells, finding low forces that do not affect bond lifetimes, suggesting the immunological synapse stabilizes antigen recognition.

The paper uses live-cell imaging to study TCR:pMHC interactions in anti-viral and autoimmunity settings, revealing differences in synaptic engagement, CD8 co-engagement, and signaling efficiency that define central tolerance boundaries.