Emeric J. Charles

This paper introduces ELXRs, CRISPR-CasX-based epigenetic repressors that use sequential proofreading to enhance specificity and potency by reading chromatin state before DNA methylation.

The paper shows that Chimalliviridae form a lipid membrane-bound EPI vesicle during early infection, which hosts transcription of the phage genome, and demonstrates the necessity of a second compartment, the phage nucleus, for genome replication.

The paper develops a CRISPRi-ART system to knock down protein expression in diverse bacteriophages and identifies essential genes, including many with unknown functions.

PopAlign identifies subpopulations and tracks gene expression changes in heterogeneous cell mixtures across drug and disease conditions.