Robert C. Spitale

This paper introduces OINC-seq, a method to quantify subcellular RNA localization through direct oxidation detection, and demonstrates its application in various cellular compartments and live zebrafish.

The paper shows DNA G-quadruplex regulates gene expression in neurons, affecting memory formation and fear extinction.

The paper identifies sequence-specific sensitivity to JTE-607 in mRNA 3' processing by analyzing over 260,000 sequence variants and developing a predictive model.

The paper identifies a lncRNA, Gas5, involved in fear extinction by regulating RNA granule trafficking and clustering at synapses in mice.

This study identifies TDP-43 dysfunction and reduced m6A modification as mechanisms causing aberrant splicing in Huntington's disease using mouse and human brain data.

The paper identifies synapse-specific m6A RNA readers and their role in fear extinction memory consolidation through RNA immunoprecipitation and sequencing in mice.

This study introduces Halo-seq, a method for quantifying subcellular RNA populations, and finds that AU-rich elements are enriched in the nucleus and their export depends on HuR.

The paper uses EC-tagging to profile md neuron transcriptomes and identifies two RNA-binding proteins involved in dendrite arborization.

This paper introduces Long-TUC-seq, a method combining metabolic labeling with long-read sequencing to reliably detect isoform-level transcription dynamics.

TALON annotates and counts full-length reads from PacBio or Nanopore in one framework; in mouse brain, 422 genes showed more novel-isoform reads than annotated ones.

The paper investigates the functional conservation of lncRNA JPX across species despite sequence and structural divergence, showing it can functionally complement Jpx in mouse cells.

This study shows that m6dA accumulation in DNA of prefrontal cortical neurons is linked to fear extinction learning and gene expression.