Alex N. T. Johnson

This paper demonstrates that a quadrupole-ion trap instrument can perform multiplexed shotgun proteomics with accuracy comparable to a dedicated tribrid instrument, offering a cost-effective alternative.

The paper demonstrates that super-resolution mass spectrometry with least-squares fitting enables highly-multiplexed, accurate, and precise shotgun proteomics at the MS2 level.

The paper quantifies protein turnover in E. coli under 12 conditions, revealing cytoplasmic protein recycling under nitrogen limitation and identifying unknown proteolysis pathways.

This paper introduces TMTProC, an optimized complementary ion quantification method for TMTPro tags, improving sensitivity and accuracy in multiplexed proteomics.