Chaoyong Huang

This paper introduces a tRNA modification-based strategy to identify amino acid overproducers in microbial hosts.

The paper modifies Escherichia coli MG1655 to create a more efficient host strain for chemical production by inactivating DNA defense systems, resulting in higher transformation efficiency and plasmid stability.

The paper demonstrates local polyploidy in E. coli through CRISPR/Cas9 deletions, showing a cell can have multiple genotypes in different replication forks, with implications for genetic engineering.