Hong Zhang

The paper identifies and characterizes non-canonical ORFs in mammals, showing their evolutionary conservation and functional integration into the proteome.

The paper evaluates four methods for detecting translated non-canonical ORFs from Ribo-Seq data and finds they have low precision and accuracy, especially for ORFs with near-cognate start codons.

PSite infers read-specific P-site offsets for ribosomal footprints, improving ORF prediction and robustness to dataset quality and cutting bias.