Joerg Bewersdorf

SPC-Clean reduces speckle and isolated pixel noise in fluorescence microscopy images using a topology-aware method without altering fluorescence intensities.

This paper describes the construction and alignment of an adaptive-optics assisted isoSTED nanoscope for achieving isotropic sub-50-nm 3D resolution in thick biological samples.

This paper introduces fluorogenic DNA-PAINT probes that improve super-resolution imaging speed and signal-to-background ratio in cells.

This paper introduces pan-ExM-t, an all-optical method for visualizing specific proteins in the ultrastructural context of brain tissue, demonstrating its application on various markers in mouse brain sections and neuron cultures.

This paper shows that renalase activates mitochondrial leak metabolism to enhance protein synthesis and protect cells from stress and injury.

The paper introduces pan-ASLM, a light sheet microscope optimized for expansion microscopy, achieving higher speed, larger field of view, and better resolution than standard confocal microscopes.

This paper introduces MAPS, a deep-learning approach for automated segmentation of mitochondrial ultrastructure in 3D pan-ExM images, enabling high-throughput quantification of morphology and protein localization.

The paper uses super-resolution microscopy to show golgin proteins form a tetraplex at the Golgi rim and self-assemble into filamentous bands, proposing a model for Golgi stack formation.

This paper identifies RAB1A and ARFGAP1-positive membranes as essential for autophagosome biogenesis, interacting with ATG2A to support lipid transport during autophagosome formation.

This study identifies VPS13B's localization at the cis-trans Golgi interface and shows its functional interaction with FAM177A1 in lipid transport and Golgi dynamics.

The paper develops a combined microscope platform that integrates quantitative phase contrast and super-resolution fluorescence imaging for high-sensitivity visualization of sub-cellular structures and molecules in their cellular context.

This paper introduces a method to enhance two-photon optogenetic stimulation in live mouse brains by using fluorescent molecules to improve opsin activation efficiency.

The paper develops a stable, high refractive index buffer for dSTORM super-resolution imaging, improving stability, switching speed, contrast, and refractive index matching.

The paper identifies dynamic nano-scale holes in ER sheets that act as reservoirs for curvature-stabilizing proteins, enabling ER tubule extension and retraction.