MicrobiologyProceedings of the National Academy of SciencesPeer-reviewed
In vivo delivery of functional Cas:DNA nucleoprotein complexes into recipient bacteria through a Type IV Secretion System
Dolores L. Guzmán-Herrador, Andrea Fernández-Gómez, Florence Depardieu…·Matxalen Llosa
This paper demonstrates the delivery of functional Cas:DNA nucleoprotein complexes into recipient bacteria via a Type IV Secretion System, enabling targeted genetic modification without requiring nuclease expression in target cells.
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