Florence Depardieu

The paper identifies and characterizes defence hotspots in P2-like phages and P4-like satellites, revealing rapid modular evolution of antiviral systems through gene swapping and recombination.

This paper characterizes OLD, an abortive immunity protein, revealing its structure and activation mechanism in response to phage replication and DNA defects in RecBCD-compromised cells.

The paper identifies additional tRNA targets of the AriB nuclease in the PARIS system and shows that phage-encoded tRNAs can inhibit this defense.

This paper demonstrates the delivery of functional Cas:DNA nucleoprotein complexes into recipient bacteria via a Type IV Secretion System, enabling targeted genetic modification without requiring nuclease expression in target cells.

The paper shows that viral proteins activate PARIS-mediated tRNA degradation, and viral tRNAs rescue infection by preventing cleavage.

The paper identifies anti-phage defence systems encoded by sedentary chromosomal integron cassettes in Vibrio cholerae, showing 16 cassettes provide phage resistance.

The paper designs novel Cas9 PAM-interacting domains using a combination of evolutionary modeling, physics-based scoring, and machine learning, identifying functional variants with up to 20% sequence divergence from the wild-type.

This paper uses variational autoencoders to generate functional protein variants of bacterial luciferase, showing high success rates in retaining activity despite significant sequence changes.