Joseph Rodriguez

This paper presents a protocol using single-molecule imaging to measure how endocrine disruptors affect transcriptional bursting in human breast cancer cells.

The paper shows that hormone response magnitude in mammary organoids and ER+ cancer cells varies independently of receptor abundance and follows distinct activation dynamics.

The paper identifies H3K27me3 chromatin heterogeneity as a mechanism underlying variable TFF1 expression and endocrine resistance in ER+ breast cancer.

The paper uses correlative single molecule lattice light sheet imaging to study nucleosome dynamics and chromatin environment interactions in live cells, revealing differential diffusion and packing with increasing chromatin density.

The paper investigates how altered ERα function and cofactor recruitment affect transcriptional bursting in estrogen responsive genes, finding gene-specific reductions in active alleles in BPA-treated cells.