Brian D. Strahl

One hour of 200 mM sorbitol collapses most chromatin loops in human cells while several hundred transient, cohesin-dependent, CTCF-independent loops form at active promoters, preceding anchor-gene activation.

This study identifies chromatin regulators of HIV latency and reactivation using RelB proximity proteomics and CRISPR screening, revealing key acetyltransferases involved in noncanonical NF-κB pathway activation.

This study shows that BRD4's tandem bromodomains cooperatively bind poly-acetylated nucleosomes, enhancing chromatin engagement and influencing breast cancer phenotypes.

This paper describes a rapid CRISPR-Cas9 genome editing protocol for S. cerevisiae using PCR-based guide installation and seamless plasmid recircularization.

The paper shows that chromatin stability prevents mTORC1 hyperactivation and mitochondrial stress during metabolic adaptation by maintaining histone integrity and regulating mitochondrial function.

This study shows SETD2 suppresses lung cancer in a KRASG12C model and its activity is regulated by histone acetylation and ubiquitination.

This study identifies a non-catalytic function of SETD2 in maintaining nuclear lamina stability and genome integrity through its N-terminus interactions.

This study shows PHRF1's PHD finger binds histone H3 N-terminal region, regulates transcription and RNA splicing, and is essential for DNA damage response.

The paper shows that H3K18 and H3K23 acetylation enhance MLL1-mediated H3K4 methylation, while other modifications are less affected by tail acetylation.

The paper shows that H3.2K4 antagonizes Polycomb group activity and promotes target gene expression in vivo.

This study shows that SETD2 inhibition reduces HIV gene expression and latency by altering viral RNA splicing, not transcription.

The paper uses correlative single molecule lattice light sheet imaging to study nucleosome dynamics and chromatin environment interactions in live cells, revealing differential diffusion and packing with increasing chromatin density.

This study identifies G9a's noncanonical role in promoting translation of genes involved in hyperinflammation and lymphopenia in COVID-19, revealing therapeutic targets that counteract viral effects.

This study shows that Set8/KMT5A and H4K20me1 have distinct roles in Drosophila development, with non-catalytic functions of Set8 and H4K20me1 contributing to viability and phenotypic differences.

This study uses optogenetic control of Set2 in yeast to show rapid, transcription-dependent H3K36me3 deposition and transcription-independent, stochastic demethylation.

This study identifies the RFTS domain of DNMT1 as a reader for H3K9me3/H3Ub, showing its role in DNA methylation maintenance and genomic stability.

The paper uses high-depth TSS mapping in D. melanogaster to identify novel initiation events and characterize enhancer-associated RNAs (eRNAs) in a developmental context.

The paper investigates the functional roles of K9 modifications on variant and canonical histone H3 in Drosophila, showing overlapping but distinct contributions to chromatin structure and gene regulation.

This study replaces H3K36 with arginine in Drosophila to show H3K36 methylation maintains transcriptome fidelity through post-transcriptional mechanisms involving polyadenylation.