Michael Lidschreiber

This study shows non-coding RNA disrupts enhancer-promoter interactions when increased, and stabilizes them when decreased, independent of transcription initiation.

The paper determines the cryo-EM structure of cytoplasmic Pol II and shows Gdown1 inhibits its transcriptional activity by blocking essential factor binding.

This study reveals the molecular mechanisms of cap-snatching by influenza polymerase using cryo-EM structures of FluPol bound to Pol II and DSIF.

The paper shows that CTCF depletion disrupts chromatin hub formation but not enhancer-promoter interactions, which still drive gene expression during differentiation.

This study shows that the Mediator complex is essential for enhancer-promoter interactions, as its depletion reduces interaction frequencies and gene expression, while increasing CTCF-binding site interactions and altering Cohesin distribution.

This study shows Oct4 primarily activates transcription of pluripotency enhancers rather than controlling their chromatin accessibility.

The paper identifies sequence determinants of human gene regulatory elements by analyzing transcriptional activity of long DNA sequences in three cell types, revealing distinct enhancer types and TF subtypes based on motif content and activity patterns.

This study uses TT-seq to analyze RNA synthesis dynamics in three mouse pluripotent states, finding reduced RNA synthesis and elongation velocity in 2i and mTORi states.

NASC-seq monitors newly synthesized RNA in single cells, revealing dynamic gene expression during T-cell activation.