Sushil Pangeni

This study shows SF1 helicase Rep translocates processively on ssDNA and, unexpectedly, also on dsDNA, with high speed and processivity, challenging existing models of helicase function.

WHaloForce is a chemigenetic tension sensor that images molecular forces in living cells and animals, revealing tissue-specific force patterns and ovulation-related molecular loading.

DNAsight automates AFM image analysis of chromatin organization, revealing protein-specific structural signatures and nucleosome spacing distributions.

The paper shows CTCF controls chromatin boundary permeability through a dynamic conformational ensemble, not static occupancy, influencing cohesin capture probabilities.

This study investigates the mechanisms of DNA and RNA strand transfer and protein exchange between SSB and RPA using smFRET, revealing dynamic interactions and length-dependent behavior.

This study reveals how Rad52 sorts and stacks Rad51 at DNA junctions to enhance homologous recombination by promoting Rad51 binding to RPA-coated ssDNA.

This study investigates RPA's long-distance migration on ssDNA using biophysical methods, finding that intersegmental transfer via multivalent interactions enables rapid diffusion under tension.