Daniele Fachinetti

This study shows that elevated MPS1 causes chromosomal instability in colon cancer by converting the fibrous corona into a source of merotelic kinetochore-microtubule attachments.

The paper identifies that canonical CENP-B boxes are highly conserved in centromeres but not in ectopic sites, showing selective conservation and functional binding.

This study shows oxidative DNA damage destabilizes centromeres, leading to chromosome instability and mitotic defects in pre-tumorigenic cells.

The paper investigates how heterochromatin boundaries maintain centromere position, size, and number through specific histone modifications and methyltransferases.

This study shows that the Arp2/3 complex is essential for genome integrity and survival of epidermal Langerhans cells, with Arpc4 deficiency leading to DNA damage and premature aging-like decline.

This study shows UHRF1 has non-canonical roles beyond DNMT1 activation in maintaining DNA methylation homeostasis in cancer cells.

This study uses tunable DNMT1 degradation to investigate DNA methylation dynamics and genome organization, revealing cooperative roles of DNMT1 and DNMT3B and their impact on heterochromatin and cell fitness.

Kronos scRT enables single-cell replication timing analysis without cell sorting, analyzing thousands of cells from mouse and human cell lines.

The paper develops a method to enrich and purify centromeric DNA from human cells, achieving 25-fold enrichment and up to 60% centromeric content in sequenced fractions.