Nigel Peter Minton

This paper develops a methylation-conjugation system for Clostridium kluyveri to enable plasmid-based genetic modification and demonstrates its use for expressing a fluorescent reporter.

The paper develops a method to create methylating conjugal donor strains to improve DNA transfer into genetically recalcitrant bacteria by mirroring their native DNA methylation signature.

This paper expands the host range of C. difficile phages by engineering their tail fibre and associated chaperone genes using CRISPR/Cas9, resulting in a phage with broader host range and higher infection efficiency.

This paper introduces an inducible CRISPR/Cas9 system for precise, markerless genome editing in Synechocystis sp. PCC 6803, enabling multiplexed edits and efficient segregation of mutants.

This paper develops methods to apply transposon-insertion sequencing in Clostridium autoethanogenum to identify essential genes for growth using carbon monoxide.

The paper introduces a test-driven framework for developing and maintaining genome-scale metabolic models, enabling continuous integration of validation data and improving model reliability.