Nisha Singh

The paper develops a genome-wide CRISPR-Cas9 knockout screening platform for Ixodes scapularis ticks and identifies genes associated with cellular fitness and drug resistance.

This study shows that T2R14 downregulates autophagy flux in gingival epithelial cells and that S. mutans CSP-1 induces calcium release dependent on T2R14 and autophagy protein 7.

This study identifies hemocyte heterogeneity and pleiotropic roles in ticks using multi-omics approaches, revealing their impact on immunity, metabolism, and arthropod fitness.

The paper characterizes T2R14-dependent autophagy in gingival epithelial cells using western blot and flow cytometry to assess autophagy flux.