Sibylle Chantal Vonesch

This paper optimizes dCas12a-based CRISPRi/a for pooled screening in Yarrowia lipolytica, identifying effective crRNA features and a high-performing effector variant.

The paper systematically analyzes Cas12a genome editing in yeast to identify design principles for efficient and stable variant engineering.

The paper identifies buffer genes in yeast that influence the phenotypic effects of mutations by modulating genetic variation's impact on fitness.

The paper develops MAGESTIC 3.0, a CRISPR-based system for genome-wide variant installation in yeast, revealing missense variants and multiple causal variants in quantitative trait loci across environmental conditions.

The paper describes a fast, low-cost method for preparing whole-genome sequencing libraries from yeast cells, enabling high-throughput analysis of genetic edits and polymorphisms.

The paper systematically maps the functional role of phosphorylation by analyzing phospho-deficient mutants in yeast and linking yeast phenotypes to human functional relevance.