Lihua Zhang

This study develops a high-throughput plasma proteome analysis workflow using UV-assisted digestion, 5-plex labeling, and MS-based high abundance protein removal to improve quantification of low-abundance proteins.

SpotLink identifies site non-specific cross-links at the proteome scale with high sensitivity and precision, outperforming existing methods on simulated and protein complexes datasets.

The paper determines the architecture of the nuclear ring in Xenopus laevis NPC using cryo-EM, homology modeling, and cell experiments, identifying key proteins and their roles in NPC assembly.