James G. Wakefield

This study develops genetic tools for Galleria mellonella, enabling transgenesis via PiggyBac and CRISPR/Cas9 gene knockout, enhancing its use as an infection model.

This paper introduces a cold-shock method to inhibit silk extrusion in Galleria mellonella larvae, improving handling for infection studies without compromising viability.

This study shows that RNase H1 reduces DNA damage and improves SMA-like symptoms in a Drosophila model by resolving RNA:DNA hybrids caused by SMN deficiency.

This paper describes a microinjection protocol for Galleria mellonella embryos to enable genetic engineering and functional studies, improving the model's utility in infection biology and 3Rs applications.

This paper develops a live flow cytometry method to analyze Galleria mellonella hemocytes and measure phagocytosis, showing 80% of hemocytes are phagocytic and demonstrating Candida albicans subverts phagocytosis.

This study identifies functional interactions between TGS1 and the Smn complex in Drosophila eye development, showing their roles in retinal progenitor cell viability and eye size.

The paper shows that mutations in the Drosophila Asp gene, which is homologous to ASPM, cause tissue-specific vulnerabilities to mitotic defects, leading to microcephaly and midgut abnormalities.