Alex J. Walsh

This study uses label-free optical imaging to compare metabolic changes in macrophages after cryopreservation versus fresh isolation, finding significant metabolic alterations post-cryopreservation.

This paper describes a protocol using NAD(P)H autofluorescence and 2-photon microscopy to image mitochondrial dynamics in 3D and over time, with results showing effective visualization of mitochondrial movement and network distribution.

This study uses FLIM to analyze metabolic changes in TNBC cells inhibited by LDHB, revealing insights into metabolic symbiosis and potential treatment strategies.

This study uses optical metabolic imaging to identify metabolic shifts and mitochondrial heterogeneity in POLG mutator macrophages, revealing increased glycolytic dependence and subpopulation variability.

The paper introduces a polarization light-sheet microscopy and tomography system for non-invasive 3D imaging of mesenchymal stem cells on microcarriers.

This study examines how propofol affects the metabolism of MDA-MB-231 breast cancer cells using fluorescence lifetime imaging microscopy.

This study compares polystyrene and gelMA microcarriers for optical imaging of MSCs, finding that gelMA allows better visualization and quantification of cells.

The paper uses autofluorescence imaging to identify and track neural stem cell activation states in vitro and in vivo, linking it to transcriptional features and cell behavior.

The paper uses convolutional neural networks to classify T cell activity from autofluorescence intensity images and shows that pre-trained models outperform traditional methods.