Author

Lynne Regan

University of EdinburghORCID 0000-0003-4719-2495Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Synthetic Biology· 4 Biochemistry· 1 Biophysics· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 6 Assay:imaging· 3 Research Focus:protein design· 2 Organism:yeast· 2 Research Focus:cell type annotation· 1 Assay:fluorescence / live-cell microscopy· 1 Organism:mouse· 1 Research Focus:protein folding & dynamics· 1

Frequent Co-Authors

Mathew H. HorrocksiD· 4 shared Zoe GiddeniD· 4 shared Curran OiiD· 3 shared Haresh BhaskariD· 3 shared Susan J. RosseriD· 3 shared Dirk‐Jan Kleinjan· 2 shared S. G. J. MochrieiD· 2 shared Ella Lucille ThorntoniD· 1 shared

Activity by Year

2020-2025 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 33 mutual co-author links

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4 shared papersSynthetic Biology

Mathew H. HorrocksORCID

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6 papers

Recent·Most cited

BiochemistryACS OmegaPeer-reviewedJul 11, 2025

A new highly specific and soluble protease for precise removal of N-terminal purification tags

Raef S. Shams, Lynne Regan

This paper describes the design and characterization of a new protease, Con1, which efficiently and specifically removes N-terminal purification tags from proteins.

1 citation

proteomicsexperimental assay

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Synthetic BiologyProtein SciencePeer-reviewedJan 25, 2025

Super-resolution imaging of proteins inside live mammalian cells with mLIVE-PAINT

Haresh Bhaskar, Zoe Gidden, Gurvir S. Virdi…·Mathew H. Horrocks

This study adapts the LIVE-PAINT method for live mammalian cells, demonstrating super-resolution imaging of two proteins in real time.

3 citations

mouseimaging

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Synthetic BiologybioRxivPreprintJan 24, 2025

Cell-free protein synthesis as a method to rapidly screen machine learning-directed protease variants

Ella Lucille Thornton, Jeremy T. Boyle, Nadanai Laohakunakorn, Lynne Regan

The paper uses cell-free protein synthesis to rapidly screen machine learning-directed protease variants and identifies improved kinetic properties in some variants.

2 citations

protein structureexperimental assay

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BiophysicsNano LettersPeer-reviewedNov 2, 2023

Imaging proteins sensitive to direct fusions using transient peptide-peptide interactions

Zoe Gidden, Curran Oi, Emily J. Johnston…·Lynne Regan

The paper introduces LIVE-PAINT, a super-resolution imaging method for live-cell visualization of proteins using transient peptide-peptide interactions, demonstrating its application on yeast membrane proteins.

8 citations

yeastfluorescence / live-cell microscopy

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Synthetic BiologyProtein SciencePeer-reviewedDec 31, 2022

Live-cell super-resolution imaging of actin using LifeAct-14 with a PAINT-based approach

Haresh Bhaskar, Dirk‐Jan Kleinjan, Curran Oi…·Lynne Regan

The paper introduces direct-LIVE-PAINT, a method for nanoscopic imaging of protein structures in live cells, demonstrating its application to actin with sub-diffraction resolution.

13 citations

imagingexperimental assay

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Synthetic BiologyCommunications BiologyPeer-reviewedAug 20, 2020

LIVE-PAINT: Super-Resolution Microscopy Inside Live Cells Using Reversible Peptide-Protein Interactions

Curran Oi, Zoe Gidden, Louise V. Holyoake…·Lynne Regan

LIVE-PAINT enables super-resolution imaging in live cells by using reversible peptide-protein interactions instead of direct fluorescent protein fusion.

62 citationstop 4%

yeastimaging

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