Matthew J. Szarzanowicz

This paper introduces a membrane-free spot-plating protocol for Agrobacterium-mediated transformation of diverse yeasts, improving efficiency and reducing costs.

This paper refactors the Agrobacterium tumor-inducing plasmid into a minimal set of genes for plant and fungal transformation, demonstrating a bottom-up engineering approach.

The paper models variability in Nicotiana benthamiana transient expression and provides guidelines for minimizing it through experimental design and power analysis.

This study shows that binary vector origin of replication family, not copy number, determines Agrobacterium-mediated transformation outcomes across plants and fungi.

This study engineers cytokinin regulators in Arabidopsis to modulate root development by tuning gene expression through synthetic biology.