Anna B. Trofimova

This paper introduces an active learning method to discover PAMs for uncharacterized CRISPR-Cas9 orthologs without sequencing, reducing data requirements and validating it on known and novel Cas9 variants.

The paper identifies and characterizes a novel type V CRISPR-Cas system, Cas12r-CRISPR, encoded by Lalka phages in Thermus bacteria, showing its role in targeting mobile genetic elements.

The paper analyzes microbiomes of three sympatric Arctic sponge species, finding functional convergence and specialization within their symbiotic communities, with shifts linked to temperature changes.

This study elucidates the molecular mechanism of BREX anti-phage immunity by showing how BrxX methyltransferase recognizes DNA and forms an immune complex to protect against phage infection.

The paper isolates and analyzes new phages infecting Thermus thermophilus from diverse hot springs, revealing both known relatives and novel phages with unique genetic features.

This study investigates the interference requirements of Type III-A and III-B CRISPR-Cas systems from Thermus thermophilus, finding that target RNA abundance influences crRNA-target complementarity needed for interference.