Author

Sophie Tschirpke

Delft University of TechnologyORCID 0009-0005-6550-9291Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Biochemistry· 2

Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Research Focus:protein folding & dynamics· 1 Assay:proteomics· 1 Organism:yeast· 1

Frequent Co-Authors

Liedewij LaaniD· 2 shared Benjamin SpitzbarthiD· 1 shared Frank van Opstal· 1 shared Mark A. R. de GeusiD· 1 shared Nynke Marije HettemaiD· 1 shared Rienk EelkemaiD· 1 shared Werner Karl-Gustav Daalman· 1 shared

Activity by Year

2024-2026 · peak 1/yr

Co-Author Collaboration Network

7 top collaborators · 16 mutual co-author links

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2 shared papersBiochemistry

Liedewij LaanORCID

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2 papers

Recent·Most cited

BiochemistryACS Synthetic BiologyPeer-reviewedMay 13, 2026

Sortase A-mediated farnesylation of Cdc42 in vitro

Sophie Tschirpke, Nynke Marije Hettema, Benjamin Spitzbarth…·Liedewij Laan

This paper introduces a Sortase A-based method to farnesylate Cdc42 in vitro, enabling efficient and reproducible prenylation for biochemical studies.

3 citations

yeastproteomics

𝕏 PostBluesky

BiochemistryCurrent ProtocolsPeer-reviewedApr 1, 2024

Quantification of GTPase cycling rates of GTPases and GTPase : effector mixtures using GTPase Glo™ assays

Sophie Tschirpke, Werner Karl-Gustav Daalman, Liedewij Laan

The paper describes the use of GTPase Glo assays to quantify GTPase cycling rates and interactions with effector proteins.

5 citations

experimental assay

𝕏 PostBluesky