Shwetha Sreenivasan

RheoScale 2.0 identifies protein positions with varied substitution outcomes, revealing roles beyond neutral or deleterious, improving interpretation of high-throughput mutagenesis data.

The paper introduces GROQ-seq, a high-throughput platform for linking protein function to sequencing data, and presents pilot datasets for transcription factors, polymerases, and proteases.

The paper uses GROQ-seq to profile function across a diverse protease library, showing robust activity across sequence divergence.

The paper experimentally separates the effects of amino acid substitutions on function and abundance for SARS-CoV-2 Mpro, revealing their distinct contributions to DMS outcomes.

This study shows that fructose-1-kinase in E. coli can reverse its reaction to generate F-1-P from F-1,6-BP, binds Cra, and influences metabolism and biofilm formation beyond fructose catabolism.

The study shows that the intrinsically disordered activation domain of CIITA is tuneable by single amino acid substitutions, challenging the acidic exposure model and suggesting variability in IDR tuneability.

The paper identifies an evolutionary pathway between two protein folds using statistical methods and structural models on bacterial response regulator proteins.