Vanessa R. Lewis

This study shows that mRNA vaccines can prime and mature ultrarare HIV bnAb precursor B cells in NHPs, with improved affinity using a modified immunogen.

DNA origami particles displaying eOD-GT8 with a synthetic T helper epitope gave antigen-focused germinal centers and expanded VRC01-class precursors in humanized mice after one immunization.

Membrane-bound BG505 MD39.3 Env trimer delivered as mRNA-LNP reduced base-directed antibody responses in rabbits and macaques and elicited neutralizing antibodies, with memory B cell responses documented in macaques.

The paper investigates how vaccine antigen delivery and adjuvant selection affect the priming of rare HIV broadly neutralizing antibody-precursor B cells in non-human primates, finding that specific dosing and adjuvant combinations enhance their detection in germinal centers.