William R. Schief

This study shows that mRNA vaccines can prime and mature ultrarare HIV bnAb precursor B cells in NHPs, with improved affinity using a modified immunogen.

This study tracks germinal center responses in rhesus macaques over a year, showing prolonged GCs with declining SHM and ongoing plasma cell production.

This paper describes the development and cGMP manufacturing of an HIV vaccine candidate for clinical trials, demonstrating scalable production and quality control.

This study uses deep learning to design multi-epitope scaffolds mimicking CSP's spatial structure, showing accurate positioning of two epitopes and partial success with the third.

DNA origami particles displaying eOD-GT8 with a synthetic T helper epitope gave antigen-focused germinal centers and expanded VRC01-class precursors in humanized mice after one immunization.

The paper investigates how B cell affinity affects germinal center (GC) responses by showing high-affinity B cells have shorter GC residence times and lower-affinity B cells are maintained, suggesting an antibody feedback mechanism that modulates affinity and epitope spreading.

This study develops and evaluates two mRNA vaccines targeting the LP.8.1 variant of SARS-CoV-2, showing broad neutralization against JN.1-lineage subvariants.

This paper uses cryoEM to analyze antibody-antigen interactions with HIV MPER-GT immunogens, revealing binding patterns and epitopes that inform antigen optimization.

This paper reports structural analysis of VRC01-class bnAb precursors from a vaccine trial, showing they bind HIV Env with germline-like features and glycan adaptability.

Membrane-bound BG505 MD39.3 Env trimer delivered as mRNA-LNP reduced base-directed antibody responses in rabbits and macaques and elicited neutralizing antibodies, with memory B cell responses documented in macaques.

This study shows that combining slow antigen delivery and follicle-targeting adjuvants enhances germinal center B cell diversity and clonal expansion in mice.

The paper presents a nanodisc platform for assembling transmembrane glycoproteins into vaccine candidates, demonstrating its utility in HIV vaccine development through structural and functional analyses.

This study shows that antigen avidity affects the durability and diversity of vaccine-induced immune responses by influencing germinal center dynamics and B cell outcomes.

The paper investigates how vaccine antigen delivery and adjuvant selection affect the priming of rare HIV broadly neutralizing antibody-precursor B cells in non-human primates, finding that specific dosing and adjuvant combinations enhance their detection in germinal centers.

This study evaluates the dose-dependent effects of the SMNP adjuvant on HIV Env-specific immune responses in non-human primates, showing increased memory T and B cell responses and neutralizing antibodies at higher doses.

This study visualizes lipid nanoparticle trafficking in non-human primates using PET imaging, showing distribution to muscle and draining lymph nodes with uptake by antigen-presenting cells.

The paper develops methodologies to generate and analyze immunoglobulin loci variation in rhesus macaques, revealing extensive diversity and expanding existing allele sets by 60%.

This study compares the immune response in non-human primates between alum and SMNP adjuvants, finding SMNP induces stronger humoral responses and enhanced lymph node activation.

The paper analyzes B cell responses to a germline-targeting immunogen in human Ig loci transgenic mice, finding diverse non-VRC01-class B cells and competitor B cells recognizing epitopes near the VRC01 epitope.

The paper presents a framework for ensuring reproducibility in bio-macromolecular modeling through automated benchmarks and a test server, demonstrating its utility for Rosetta and other tools.

This paper expands the Rosetta software suite with tools for glycan modeling, density fitting, and rational sequon design, achieving high accuracy in predicting and building glycan structures.