A high-quality de novo genome assembly of Asian Crested Ibis (Nipponia Nippon) using long-read and Hi-C data

Youngseok Yu; Sung-jin Kim; Changhan Yoon; Jihun Bhak; Changjae Kim; Hyebin Park; Younghui Kang; Yeonkyung Kim; Yujin Lee; Seung-yeon Kang; Yong-un Shin; Jong Bhak; Sungwon Jeon

doi:10.1101/2024.04.29.591545

BioinformaticsbioRxivApr 29, 2024

Preprint

A high-quality de novo genome assembly of Asian Crested Ibis (Nipponia Nippon) using long-read and Hi-C data

Youngseok Yu, Sung-jin Kim, Changhan Yoon iD, Jihun Bhak iD, Changjae Kim iD, Hyebin Park iD, Younghui Kang iD, Yeonkyung Kim iD, Yujin Lee iD, Seung-yeon Kang, Yong-un Shin, Jong Bhak iD, Sungwon Jeon iD

Abstract

We present TtaoRef1, the highest-quality de novo genome assembly of Asian Crested Ibis (Nipponia Nippon) to date consisting of 134 scaffolds with a length of 1.25 Gb and N50 of 101,183,595 bp. This assembly was generated through the utilization of long-read sequencing and Hi-C data. The assessment of assembly quality, conducted via Benchmarking Universal Single-Copy Orthologs (BUSCO), revealed the presence of 96.8% of completely predicted single-copy genes. TtaoRef1 had 18 times longer N50 value than the previous assembly (ASM70822v1), Furthermore, we conducted the annotation of 24,681 protein-coding genes within the newly assembled genome sequences.

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