Horizontally transferred cell-free chromatin particles function as autonomous satellite genomes and vehicles for transposable elements within host cells

Abstract

BackgroundHorizontal gene transfer (HGT) plays an important evolutionary role in prokaryotes, but it is thought to be less frequent in mammals. We previously reported that cell-free chromatin particles (cfChPs) - chromosomal fragments released from the billions of dying cells that circulate in human blood - are horizontally transferred to healthy cells with biological effects. However, the underlying mechanism and function of these effects remained unclear. MethodsWe treated NIH3T3 mouse fibroblasts cells with cfChPs isolated from human serum and serially passaged the cells. The intracellular activities of cfChPs were analysed using chromatin fibre fluorography, cytogenetic analysis, immuno-fluorescence and fluorescent in situ hybridisation. ResultsWe discovered that the internalised cfChPs were almost exclusively comprised of non-coding DNA, and the disparate DNA sequences contained within them had randomly combined to form complex concatemers some of which were ostensibly multi-mega base pairs in size. The concatemers exhibited variable and bizarre spatial relationships with the host cell interphase DNA with many remaining in the cytoplasm and others aligning themselves with the mouse chromosomal DNA. The concatemers autonomously performed many functions attributable to the nuclear genome. Being associated with DNA polymerase, the concatemers could synthesize DNA and autonomously replicate themselves without heed to the mitotic cycle of the host cell. They could synthesise RNA, RNA polymerase, ribosomal RNA, ribosomal proteins, and generate numerous human proteins, including oncogenes, within the mouse cells which manifested as complex and highly amplified multi-peptide fusion proteins. The concatemers harboured human LINE-1 and Alu elements, which being associated with DNA polymerase, reverse transcriptase and transposes could markedly amplify themselves and increase their copy number with time in culture with the potential to rearrange themselves within the mouse genome. The above findings were reproducible in four other cell lines derived from different species suggesting that horizontal transfer of cfChPs may be a universal phenomenon. ConclusionsOur results lead us to propose that: 1) a cell simultaneously harbours two autonomous genome forms: one that is inherited (hereditary genome) and numerous others that are acquired (satellite genomes); 2) satellite genomes may potentially have evolutionary functions given their ability to serve as vehicles for transposable elements and to generate a plethora of novel proteins; 3) transposable elements are "foreign" genetic elements that are acquired from dying cells via HGT; 4) non-coding DNA has many hidden biological functions that remain dormant but are activated following cellular apoptosis to become detectable in association with the cfChP concatemers; 5) "within-self" HGT occurs in mammals on a massive scale via the medium of cfChP concatemers that have undergone extensive and complex modifications resulting in their behaviour as "foreign" genetic elements. Video Abstracthttps://drive.google.com/file/d/1I6NbrYmT9BRM63ywYj47AU0O9WJ_NrcR/view?usp=drive_link