RhoBAST - A Rhodamine-Binding Aptamer for Super-Resolution RNA Imaging
M. Sunbul, J. Lackner, A. Martin, D. Englert, B. Hacene, K. Nienhaus, G. U. Nienhaus, A. Jäschke
Abstract
RhoBAST is a novel fluorescence light-up RNA aptamer (FLAP) that transiently binds a fluorogenic rhodamine dye. Fast dye association and dissociation result in intermittent fluorescence emission, facilitating single-molecule localization microscopy (SMLM) with an image resolution not limited by photobleaching. We demonstrate RhoBAST's excellent properties as a RNA marker by resolving subcellular and subnuclear structures of RNA in live and fixed cells by SMLM and structured illumination microscopy (SIM).
02 /Specification & Classification
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