CRISPR-Tag: an Efficient DNA Tagging System in Living Cells
Abstract
Main textA lack of efficient tools to image non-repetitive genes in living cells has limited our ability to explore the functional impact of spatiotemporal dynamics of genes. Here, we addressed this issue by developing the CRISPR-Tag system as a new DNA tagging strategy to label protein-coding genes with high signal-to-noise ratio under wild-field fluorescence microscopy by using 1 to 4 highly active sgRNAs. The CRISPR-Tag, with minimal size of [~] 250 bp, represents an easily and broadly applicable technique to study spatiotemporal organization of genomic elements in living cells.
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