High throughput single cell sequencing of both T-cell-receptor-beta alleles
Tomonori Hosoya, Hongyang LiiD, Chia-Jui Ku, Qingqing WuiD, Yuanfang GuaniD, James Douglas Engel
Abstract
Allelic exclusion is a vital mechanism for the generation of monospecificity to foreign antigens in B- and T-lymphocytes. Here we developed a high-throughput barcoded method to simultaneously analyze the VDJ recombination status of both mouse T cell receptor beta alleles in hundreds of single cells using Next Generation Sequencing.
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