RNA Immunoprecipitation and Sequencing of Isolated RNAs (RIP-SIR) Identifies Endogenous miRNA-Target Interactions

Julie M. Wells; Anuj Srivastava; Richard S. Maser; Rupesh K. Kesharwani; Vishal Sarsani; Sandra L Daigle; Ryan P. Lynch; Heidi J. Munger; Rosalinda A. Doty; Ruth L. Saxl; Carol J. Bult

doi:10.1101/2022.08.22.504764

Molecular BiologybioRxivAug 22, 2022

Preprint

RNA Immunoprecipitation and Sequencing of Isolated RNAs (RIP-SIR) Identifies Endogenous miRNA-Target Interactions

Julie M. Wells iD, Anuj Srivastava iD, Richard S. Maser, Rupesh K. Kesharwani iD, Vishal Sarsani iD, Sandra L Daigle, Ryan P. Lynch, Heidi J. Munger, Rosalinda A. Doty, Ruth L. Saxl, Carol J. Bult iD

Abstract

MicroRNAs (miRNAs) are crucial post-transcriptional regulators of gene expression in species ranging from plants to mammals. Unraveling the complex networks of miRNA-mediated regulation of gene expression requires the ability to identify the mRNA targets of individual miRNAs in native cellular contexts. Here we describe RIP-SIR, an unbiased genome-wide method for identifying interactions between endogenous miRNAs and their targets in almost any tissue or cell type.

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