Ligand binding characteristics of an NAD+ riboswitch revealed by FRET and biolayer interferometry
Abstract
The Class II NAD+ riboswitch is a bacterial RNA that binds ligands containing nicotinamide. Herein, we report a fluorescence and biolayer interferometry study of riboswitch interactions with {beta}-NMN. The results reveal a shift in the prevalence of a pseudoknot structure in the presence of ligand and Mg2+.
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