Identification of Inhibitors of Chikungunya virus nsP2 ATPase

Hernán A. Navarro; John E. Scott; Ginger R. Smith; Pegah Ghiabi; Elisa Gibson; P. Loppnau; Rachel Harding; Mohammad Anwar Hossain; M. Bose; Kenneth H. Pearce; Eric M. Merten; Timothy Mark Willson; Peter J. Brown

doi:10.1101/2024.12.02.625520

BiochemistrybioRxivDec 2, 2024

Preprint

Identification of Inhibitors of Chikungunya virus nsP2 ATPase

Hernán A. Navarro, John E. Scott, Ginger R. Smith iD, Pegah Ghiabi, Elisa Gibson iD, P. Loppnau iD, Rachel Harding iD, Mohammad Anwar Hossain iD, M. Bose iD, Kenneth H. Pearce iD, Eric M. Merten iD, Timothy Mark Willson iD, Peter J. Brown iD

Abstract

Non-structural protein 2 (nsP2), which plays an essential role in replication of CHIKV, contains a protease, helicase, and methyltransferase-like domain. We executed a simple a screen using malachite green to detect compounds that decreased ATP hydrolysis and tested a library of diverse compounds to find inhibitors of CHIKV nsP2 helicase.

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